Clonal diversity of ESBL-producing Escherichia coli in pigs at slaughter level in Portugal.
Foodborne Pathog Dis
; 10(1): 74-9, 2013 Jan.
Article
em En
| MEDLINE
| ID: mdl-23186548
ABSTRACT
We aimed to determine the prevalence of extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli in fecal samples of healthy pigs, and to evaluate their clonality and associated resistance. Forty-nine percent of pigs sampled (n=35/71) in a slaughterhouse in Portugal revealed ESBL-producing E. coli isolates. Most isolates produced CTX-M-1 enzyme (71.4%; n=25/35), followed by CTX-M-9 (11.4%; n=4/35), CTX-M-14 (5.7%; n=2/35), SHV-12 (5.7%; n=2/35), and CTX-M-32 (5.7%; n=2/35). Ninety-four percent of the isolates presented a phenotype of multi-resistance. Most isolates belonged to phylogroups B1 (42.8%; n=15/35) and A (40%; n=14/35). Multilocus sequence typing (MLST) analysis revealed nine sequence types (STs) under six clonal complexes (CCs) and nine singletons, including overrepresentation of CC10 and three new STs (ST2524, ST2525, ST2528). We observed the frequent presence of CTX-M-producing E. coli in pigs at slaughter level, most of them belonging to CC10, commonly recovered from clinical samples.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Doenças dos Suínos
/
Variação Genética
/
Beta-Lactamases
/
Portador Sadio
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Escherichia coli
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Infecções por Escherichia coli
Tipo de estudo:
Prevalence_studies
/
Risk_factors_studies
Limite:
Animals
País como assunto:
Europa
Idioma:
En
Ano de publicação:
2013
Tipo de documento:
Article