Crystallization and preliminary X-ray diffraction analysis of mevalonate kinase from Methanosarcina mazei.
Acta Crystallogr Sect F Struct Biol Cryst Commun
; 68(Pt 12): 1560-3, 2012 Dec 01.
Article
em En
| MEDLINE
| ID: mdl-23192048
ABSTRACT
Mevalonate kinase (MVK), which plays an important role in catalysing the biosynthesis of isoprenoid compounds derived from the mevalonate pathway, transforms mevalonate to 5-phosphomevalonate using ATP as a cofactor. Mevalonate kinase from Methanosarcina mazei (MmMVK) was expressed in Escherichia coli, purified and crystallized for structural analysis. Diffraction-quality crystals of MmMVK were obtained by the vapour-diffusion method using 0.32â
M MgCl2, 0.08â
M bis-tris pH 5.5, 16%(w/v) PEG 3350. The crystals belonged to space group P2(1)2(1)2, with unit-cell parameters a=97.11, b=135.92, c=46.03â
Å. Diffraction data were collected to 2.08â
Å resolution.
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Base de dados:
MEDLINE
Assunto principal:
Methanosarcina
/
Fosfotransferases (Aceptor do Grupo Álcool)
Idioma:
En
Ano de publicação:
2012
Tipo de documento:
Article