[Meiotic analysis of spermatogenic cells in severe oligoasthenoteratozoospermia with chromosome 13 rearrangement].
Zhonghua Nan Ke Xue
; 18(9): 793-6, 2012 Sep.
Article
em Zh
| MEDLINE
| ID: mdl-23193665
ABSTRACT
OBJECTIVE:
To explore the possible mechanisms of spermatogenic arrest in severe oligoasthenoteratozoospermia induced by supernumerary, ring-neocentric 13q12.3 --> 13q22 chromosome and reciprocal deletion.METHODS:
We performed a genomic-wide high-density oaCGH analysis for a case of oligoasthenoteratozoospermia with abnormal chromosome 13 to characterize the breakpoints of the chromosome involved or the gene deletion caused by the rearrangement. We also conducted a fluorescence in situ hybridization analysis on the germ cells using probes of 13q14/13qter to observe the pairing condition of homologous chromosome 13.RESULTS:
We identified by oaCGH analysis a microdeletion of 4 consecutive probes (A_16_P19757882, A_16_P02744617, A_14_ P108858 and A_16_P02744687 at chr13q12.3 27979261 --> 28039191) with 59.93 kb between the FLT1 and POMP genes, with no annotated genes in the deleted region. The signals of 13q14 and 13qter were separated from each other in 90% of all the primary spermatocytes examined, indicating the unpairing of homologous chromosome 13 or synapse failure.CONCLUSION:
Chromosomal rearrangement-induced spermatogenesis failure is caused by the unpairing of the homologous chromosomes involved in the first meiotic division of germ cells.
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Base de dados:
MEDLINE
Assunto principal:
Oligospermia
/
Cromossomos Humanos Par 13
/
Aberrações Cromossômicas
/
Astenozoospermia
/
Meiose
Limite:
Adult
/
Humans
/
Male
Idioma:
Zh
Ano de publicação:
2012
Tipo de documento:
Article