Hierarchical model of fibrillar collagen organization for interpreting the second-order susceptibility tensors in biological tissue.
Biophys J
; 103(10): 2093-105, 2012 Nov 21.
Article
em En
| MEDLINE
| ID: mdl-23200043
ABSTRACT
The second-order nonlinear polarization properties of fibrillar collagen in various rat tissues (vertebrae, tibia, tail tendon, dermis, and cornea) are investigated with polarization-dependent second-harmonic generation (P-SHG) microscopy. Three parameters are extracted the second-order susceptibility ratio, R = [Formula see text] ; a measure of the fibril distribution asymmetry, |A|; and the weighted-average fibril orientation, <δ>. A hierarchical organizational model of fibrillar collagen is developed to interpret the second-harmonic generation polarization properties. Highlights of the model include collagen type (e.g., type-I, type-II), fibril internal structure (e.g., straight, constant-tilt), and fibril architecture (e.g., parallel fibers, intertwined, lamellae). Quantifiable differences in internal structure and architecture of the fibrils are observed. Occurrence histograms of R and |A| distinguished parallel from nonparallel fibril distributions. Parallel distributions possessed low parameter values and variability, whereas nonparallel distributions displayed an increase in values and variability. From the P-SHG parameters of vertebrae tissue, a three-dimensional reconstruction of lamellae of intervertebral disk is presented.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Especificidade de Órgãos
/
Modelos Moleculares
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Colágenos Fibrilares
Limite:
Animals
Idioma:
En
Ano de publicação:
2012
Tipo de documento:
Article