Isolation of a human vimentin cDNA with a long 3'-noncoding region from a human osteosarcoma cell line (MG-63).

ABSTRACT

Human vimentin cDNA clones were isolated and sequenced from a lambda gt11 library of the human osteosarcoma cell line MG-63. The 3'-noncoding region of the sequence is 325 nucleotides (nt) long. This cDNA sequence includes both the further 104 nt and the polyadenylation signal site at the 3' end which were earlier predicted from the genomic nt sequence, but had not been found in any previously cloned cDNAs.