Negevirus: a proposed new taxon of insect-specific viruses with wide geographic distribution.
J Virol
; 87(5): 2475-88, 2013 Mar.
Article
em En
| MEDLINE
| ID: mdl-23255793
ABSTRACT
Six novel insect-specific viruses, isolated from mosquitoes and phlebotomine sand flies collected in Brazil, Peru, the United States, Ivory Coast, Israel, and Indonesia, are described. Their genomes consist of single-stranded, positive-sense RNAs with poly(A) tails. By electron microscopy, the virions appear as spherical particles with diameters of â¼45 to 55 nm. Based on their genome organization and phylogenetic relationship, the six viruses, designated Negev, Ngewotan, Piura, Loreto, Dezidougou, and Santana, appear to form a new taxon, tentatively designated Negevirus. Their closest but still distant relatives are citrus leposis virus C (CiLV-C) and viruses in the genus Cilevirus, which are mite-transmitted plant viruses. The negeviruses replicate rapidly and to high titer (up to 10(10) PFU/ml) in mosquito cells, producing extensive cytopathic effect and plaques, but they do not appear to replicate in mammalian cells or mice. A discussion follows on their possible biological significance and effect on mosquito vector competence for arboviruses.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Phlebotomus
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Vírus de RNA
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Culex
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Vírus de Insetos
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Anopheles
Limite:
Animals
Idioma:
En
Ano de publicação:
2013
Tipo de documento:
Article