The chromatin remodeler p400 ATPase facilitates Rad51-mediated repair of DNA double-strand breaks.
J Cell Biol
; 199(7): 1067-81, 2012 Dec 24.
Article
em En
| MEDLINE
| ID: mdl-23266955
ABSTRACT
DNA damage signaling and repair take place in a chromatin context. Consequently, chromatin-modifying enzymes, including adenosine triphosphate-dependent chromatin remodeling enzymes, play an important role in the management of DNA double-strand breaks (DSBs). Here, we show that the p400 ATPase is required for DNA repair by homologous recombination (HR). Indeed, although p400 is not required for DNA damage signaling, DNA DSB repair is defective in the absence of p400. We demonstrate that p400 is important for HR-dependent processes, such as recruitment of Rad51 to DSB (a key component of HR), homology-directed repair, and survival after DNA damage. Strikingly, p400 and Rad51 are present in the same complex and both favor chromatin remodeling around DSBs. Altogether, our data provide a direct molecular link between Rad51 and a chromatin remodeling enzyme involved in chromatin decompaction around DNA DSBs.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
DNA Helicases
/
Proteínas de Ligação a DNA
/
Rad51 Recombinase
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Quebras de DNA de Cadeia Dupla
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Reparo de DNA por Recombinação
Tipo de estudo:
Prognostic_studies
Limite:
Humans
Idioma:
En
Ano de publicação:
2012
Tipo de documento:
Article