Gastrointestinal permeability in patients with irritable bowel syndrome assessed using a four probe permeability solution.
Clin Chim Acta
; 418: 97-101, 2013 Mar 15.
Article
em En
| MEDLINE
| ID: mdl-23328210
ABSTRACT
BACKGROUND:
Abnormal gastrointestinal permeability has been linked to irritable bowel syndrome (IBS). The lactulose-to-mannitol ratio is traditionally used to assess small intestine permeability while sucralose and sucrose are used to assess colonic and gastric permeability respectively. We used a single 4-probe test solution to assess permeability throughout the gastrointestinal tract in IBS patients and healthy controls by measuring the recovery of the probes in urine after ingestion using a modified liquid chromatography mass spectrometry protocol.METHODS:
Fasting participants (N=59) drank a permeability test solution (100ml sucralose, sucrose, mannitol, and lactulose). Urine was collected over a 5-h period and kept frozen until analysis. Urinary sugar concentrations were measured using a liquid chromatography/triple quadruple mass spectrometer.RESULTS:
Colonic permeability was significantly lower in IBS patients when compared to healthy controls (p=0.011). Gastric and small intestinal permeability did not significantly differ between the groups.CONCLUSIONS:
The study demonstrates the clinical potential of this non-invasive method for assessing alterations in gastrointestinal permeability in patients with IBS.
Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Sacarose
/
Trato Gastrointestinal
/
Síndrome do Intestino Irritável
/
Lactulose
/
Manitol
Tipo de estudo:
Guideline
Limite:
Adult
/
Female
/
Humans
/
Male
Idioma:
En
Ano de publicação:
2013
Tipo de documento:
Article