[R954 mutations in KIF21A gene in Chinese patients with congenital fibrosis of extraocular muscles].
Zhonghua Yan Ke Za Zhi
; 48(12): 1077-82, 2012 Dec.
Article
em Zh
| MEDLINE
| ID: mdl-23336411
ABSTRACT
OBJECTIVE:
Screening KIF21A gene mutation in 9 families with congenital fibrosis of extraocular muscles and 7 sporadic cases.METHODS:
Families were ascertained and patients underwent complete ophthalmological examinations. The probands of 9 families with CFEOM and 7 sporadic patients were recruited for this study after informed consent. Genomic DNA was isolated from 5 ml peripheral blood samples according to the standard methods. Direct sequencing was performed after PCR amplification to genomic DNA for detection of KIF21A gene mutation.RESULTS:
We identified heterozygous KIF21A mutations in 14 of sixteen patients. Twelve of them harbor the most common mutation, c.2860C > T (p.R954W) and two of them harbor the second most common mutation, c2861G > A(p.R954Q). The R954 mutations account for 87.5% (14/16), in which 75% (12/16) are R954W, 12.5% (2/16) are R954Q.CONCLUSION:
The R954 mutations are also hotspots in Chinese patients with CFEOM.
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Base de dados:
MEDLINE
Assunto principal:
Transtornos da Motilidade Ocular
/
Cinesinas
Limite:
Female
/
Humans
/
Male
Idioma:
Zh
Ano de publicação:
2012
Tipo de documento:
Article