Species identification of medically important trematodes in aquatic food samples using PCR-RFLP targeting 18S rRNA.
Foodborne Pathog Dis
; 10(3): 290-2, 2013 Mar.
Article
em En
| MEDLINE
| ID: mdl-23379283
ABSTRACT
The authors describe a polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method targeting the 18S rRNA gene for the species detection of medically important trematode infecting fish and oysters, and suggest that this PCR-RFLP method based on a specific Tre-18 primer and the restriction enzymes, Acc1, Ava2, Msp1, and Hinf1, is useful for the detection of parasites in aquatic food.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Polimorfismo de Fragmento de Restrição
/
RNA Ribossômico 18S
/
Reação em Cadeia da Polimerase
/
DNA de Helmintos
Tipo de estudo:
Diagnostic_studies
Limite:
Animals
País como assunto:
Asia
Idioma:
En
Ano de publicação:
2013
Tipo de documento:
Article