An in vitro-selected RNA receptor for the GAAC loop: modular receptor for non-GNRA-type tetraloop.
Nucleic Acids Res
; 41(6): 3748-59, 2013 Apr 01.
Article
em En
| MEDLINE
| ID: mdl-23382175
ABSTRACT
Although artificial RNA motifs that can functionally replace the GNRA/receptor interaction, a class of RNA-RNA interacting motifs, were isolated from RNA libraries and used to generate designer RNA structures, receptors for non-GNRA tetraloops have not been found in nature or selected from RNA libraries. In this study, we report successful isolation of a receptor motif interacting with GAAC, a non-GNRA tetraloop, from randomized sequences embedded in a catalytic RNA. Biochemical characterization of the GAAC/receptor interacting motif within three structural contexts showed its binding affinity, selectivity and structural autonomy. The motif has binding affinity comparable with that of a GNRA/receptor, selectivity orthogonal to GNRA/receptors and structural autonomy even in a large RNA context. These features would be advantageous for usage of the motif as a building block for designer RNAs. The isolated motif can also be used as a query sequence to search for unidentified naturally occurring GANC receptor motifs.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
RNA
Tipo de estudo:
Clinical_trials
Idioma:
En
Ano de publicação:
2013
Tipo de documento:
Article