Construction of a bovine enterovirus-based vector expressing a foot-and-mouth disease virus epitope.
J Virol Methods
; 189(1): 101-4, 2013 Apr.
Article
em En
| MEDLINE
| ID: mdl-23391822
A recombinant infectious bovine enterovirus (BEV) vector was constructed to express a foot-and-mouth disease virus (FMDV) capsid protein (VP1) epitope. Sequences encoding the VP1 epitope (amino acid residues 141-160) of FMDV (vaccine strain O1/Manisa/Turkey/69) were inserted into pBLUBEV at the VP1/2A junction. The growth characteristics of the parental virus and viruses derived from recombinant plasmids (pBLUBEV, pBLUBEV-Manisa-epi) were determined by plaque assay and one-step growth curve analysis. There were no significant differences in the growth kinetics and plaque morphologies between transfectant viruses and their parental virus. The expressed VP1 epitope was detected successfully by using indirect immunofluorescence assay with a polyclonal antibody against the FMDV VP1 epitope from Madin Darby bovine kidney (MDBK) cells infected with BEV-Manisa-epi transfectant virus. This study demonstrated a novel alternative live viral vector that may be utilized as a candidate vaccine vector for veterinary applications.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Técnica Indireta de Fluorescência para Anticorpo
/
Vírus da Febre Aftosa
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Enterovirus Bovino
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Proteínas do Capsídeo
Limite:
Animals
Idioma:
En
Ano de publicação:
2013
Tipo de documento:
Article