Directed organization of DNA filaments in a soft matter template.
Langmuir
; 29(10): 3398-403, 2013 Mar 12.
Article
em En
| MEDLINE
| ID: mdl-23425153
ABSTRACT
We have developed a noninvasive, all-optical, holographic technique for permanently aligning liquid crystalline DNA filaments in a microperiodic template realized in soft-composite (polymeric) materials. By combining optical intensity holography with a selective microfluidic etching process, a channelled microstructure has been realized which enables self-assembly of DNA. The striking chemicophysical properties of the structure immobilize the DNA filaments within the microchannels without the need of any kind of surface chemistry or functionalization. Polarized optical, confocal, and electronic microscopies have been used for characterizing the DNA geometry inside the microchannels in terms of birefringence, fluorescence, and nanoscale organization properties. In particular, observation of a far-field diffraction pattern confirms a periodic organization of the DNA filaments inside the polymeric template.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Polímeros
/
DNA
Idioma:
En
Ano de publicação:
2013
Tipo de documento:
Article