Comparative proteomic study between human normal motility sperm and idiopathic asthenozoospermia.
World J Urol
; 31(6): 1395-401, 2013 Dec.
Article
em En
| MEDLINE
| ID: mdl-23455884
ABSTRACT
PURPOSE:
Idiopathic asthenozoospermia is considered as one of the causes of male infertility and characterized by reduced sperm motility. For a better determination of pathogenic mechanism of asthenozoospermia, the exploration of differentially expressed proteins in normal sperm motility and idiopathic asthenozoospermia was conducted in our study.METHODS:
Sperm proteins were extracted and isolated by two-dimensional electrophoresis. All significantly changed protein spots were picked up from 2D gels and identified by tandem mass spectrometry. Sixteen of the thirty-three total differentially expressed protein spots were successfully identified by matrix-assisted laser desorption/ionization time-of-flight tandem mass spectrometry.RESULTS:
Sixteen proteins identified belonged to 15 unique protein groups. GRP78, lactoferrin, SPANXB, PGK2, flagellin, DJ-1, XPA binding protein 2, CAB2, GPX4, and GAPDH were the first to be identified as differentially expressed proteins in idiopathic asthenospermia patients. Meanwhile, the analysis of quantitative RT-PCR was carried out to compare the protein levels, and the results indicated that the expression levels of the gene and protein were not entirely consistent.CONCLUSIONS:
These experimental results expand the scope of the protein database, generating targets for further investigation of the pathogenic mechanism of idiopathic asthenozoospermia.
Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Motilidade dos Espermatozoides
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Espermatozoides
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Regulação da Expressão Gênica
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Proteômica
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Astenozoospermia
Tipo de estudo:
Observational_studies
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Prognostic_studies
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Risk_factors_studies
Idioma:
En
Ano de publicação:
2013
Tipo de documento:
Article