Isolation of monodisperse nanodisc-reconstituted membrane proteins using free flow electrophoresis.
Anal Chem
; 85(7): 3497-500, 2013 Apr 02.
Article
em En
| MEDLINE
| ID: mdl-23458128
ABSTRACT
Free flow electrophoresis is used for rapid and high-recovery isolation of homogeneous preparations of functionally active membrane proteins inserted into nanodiscs. The approach enables isolation of integral and membrane anchored proteins and is also applicable following introduction of, e.g., fluorescent tags. Preparative separation of membrane protein loaded nanodiscs from empty nanodiscs and protein aggregates results in monodisperse nanodisc preparations ideal for structural and functional characterization using biophysical methods.
Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Proteínas de Plantas
/
Arabidopsis
/
Sorghum
/
Eletroforese
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Proteínas de Membrana
Idioma:
En
Ano de publicação:
2013
Tipo de documento:
Article