Structural features and bioremediation activity of an exopolysaccharide produced by a strain of Enterobacter ludwigii isolated in the Chernobyl exclusion zone.
Carbohydr Polym
; 93(1): 154-62, 2013 Mar 01.
Article
em En
| MEDLINE
| ID: mdl-23465914
ABSTRACT
The bacterium Enterobacter ludwigii Ez-185-17, member of the family Enterobacteriaceae, was isolated from the root nodules of plants harvested in the nuclear power region of Chernobyl. Under batch culture conditions, the bacteria produce a high-molecular-mass exopolysaccharide (EPS). After purification, the structure of this EPS was determined using a combinatory approach including monosaccharide composition (GC-FID, HPAEC-PAD) and branching structure determination (GC-MS), as well as 1D/2D NMR ((1)H, (13)C) and ESI-MS (HR, MS/MS) studies of oligosaccharides obtained from mild acid hydrolysis. The EPS was found to be a charged hexasaccharide with a repeating unit composed of d-galactose, d-glucose, l-fucose, d-glucuronic acid (2121) and substituted with acyl and pyruvyl groups. The metal-binding properties of the exopolysaccharide were then investigated, and the results seem to indicate that the EPS decreased Cd sequestration in flax seeds.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Polissacarídeos Bacterianos
/
Biodegradação Ambiental
/
Acidente Nuclear de Chernobyl
/
Enterobacter
Idioma:
En
Ano de publicação:
2013
Tipo de documento:
Article