Cell-laden hydrogels in integrated microfluidic devices for long-term cell culture and tubulogenesis assays.
Small
; 9(18): 3076-81, 2013 Sep 23.
Article
em En
| MEDLINE
| ID: mdl-23468408
ABSTRACT
A hydrogel biochip combining microfluidic mixing and orthogonal supplementation strategies is developed and validated to allow facile generation of libraries of optically transparent 3D culture microenvironments. Live, on-chip tracing of embryonic stem cell differentiation and endothelial cell tubulogenesis confirms that the platform can be used to both create communities of discrete 3D microenvironments as well as to locally monitor subsequent divergent responses at both single cell and multi-cell scales.
Palavras-chave
Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Técnicas de Cultura de Células
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Hidrogéis
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Técnicas Analíticas Microfluídicas
Limite:
Animals
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Humans
Idioma:
En
Ano de publicação:
2013
Tipo de documento:
Article