QuaNCAT: quantitating proteome dynamics in primary cells.
Nat Methods
; 10(4): 343-6, 2013 Apr.
Article
em En
| MEDLINE
| ID: mdl-23474466
ABSTRACT
Here we demonstrate quantitation of stimuli-induced proteome dynamics in primary cells by combining the power of bio-orthogonal noncanonical amino acid tagging (BONCAT) and stable-isotope labeling of amino acids in cell culture (SILAC). In conjunction with nanoscale liquid chromatography-tandem mass spectrometry (nanoLC-MS/MS), quantitative noncanonical amino acid tagging (QuaNCAT) allowed us to monitor the early expression changes of >600 proteins in primary resting T cells subjected to activation stimuli.
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Base de dados:
MEDLINE
Assunto principal:
Linfócitos T CD4-Positivos
/
Regulação da Expressão Gênica
/
Proteômica
Tipo de estudo:
Diagnostic_studies
Limite:
Humans
Idioma:
En
Ano de publicação:
2013
Tipo de documento:
Article