Integrated view of genome structure and sequence of a single DNA molecule in a nanofluidic device.
Proc Natl Acad Sci U S A
; 110(13): 4893-8, 2013 Mar 26.
Article
em En
| MEDLINE
| ID: mdl-23479649
ABSTRACT
We show how a bird's-eye view of genomic structure can be obtained at â¼1-kb resolution from long (â¼2 Mb) DNA molecules extracted from whole chromosomes in a nanofluidic laboratory-on-a-chip. We use an improved single-molecule denaturation mapping approach to detect repetitive elements and known as well as unique structural variation. Following its mapping, a molecule of interest was rescued from the chip; amplified and localized to a chromosome by FISH; and interrogated down to 1-bp resolution with a commercial sequencer, thereby reconciling haplotype-phased chromosome substructure with sequence.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
DNA
/
Genoma Humano
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Mapeamento Cromossômico
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Cromossomos Humanos
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Técnicas Analíticas Microfluídicas
Limite:
Humans
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Male
Idioma:
En
Ano de publicação:
2013
Tipo de documento:
Article