RNA-based fluorescent biosensors for live cell imaging of second messengers cyclic di-GMP and cyclic AMP-GMP.
J Am Chem Soc
; 135(13): 4906-9, 2013 Apr 03.
Article
em En
| MEDLINE
| ID: mdl-23488798
ABSTRACT
Cyclic dinucleotides are an important class of signaling molecules that regulate a wide variety of pathogenic responses in bacteria, but tools for monitoring their regulation in vivo are lacking. We have designed RNA-based fluorescent biosensors for cyclic di-GMP and cyclic AMP-GMP by fusing the Spinach aptamer to variants of a natural GEMM-I riboswitch. In live cell imaging experiments, these biosensors demonstrate fluorescence turn-on in response to cyclic dinucleotides, and they were used to confirm in vivo production of cyclic AMP-GMP by the enzyme DncV.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
RNA
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Técnicas Biossensoriais
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AMP Cíclico
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GMP Cíclico
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Imagem Molecular
Idioma:
En
Ano de publicação:
2013
Tipo de documento:
Article