Neurokinin-1 receptor signalling impacts bone marrow repopulation efficiency.
PLoS One
; 8(3): e58787, 2013.
Article
em En
| MEDLINE
| ID: mdl-23516556
ABSTRACT
Tachykinins are a large group of neuropeptides with both central and peripheral activity. Despite the increasing number of studies reporting a growth supportive effect of tachykinin peptides in various in vitro stem cell systems, it remains unclear whether these findings are applicable in vivo. To determine how neurokinin-1 receptor (NK-1R) deficient hematopoietic stem cells would behave in a normal in vivo environment, we tested their reconstitution efficiency using competitive bone marrow repopulation assays. We show here that bone marrow taken from NK-1R deficient mice (Tacr1(-/-)) showed lineage specific B and T cell engraftment deficits compared to wild-type competitor bone marrow cells, providing evidence for an involvement of NK-1R signalling in adult hematopoiesis. Tachykinin knockout mice lacking the peptides SP and/or HK-1 (Tac1 (-/-), Tac4 (-/-) and Tac1 (-/-)/Tac4 (-/-) mice) repopulated a lethally irradiated wild-type host with similar efficiency as competing wild-type bone marrow. The difference between peptide and receptor deficient mice indicates a paracrine and/or endocrine mechanism of action rather than autocrine signalling, as tachykinin peptides are supplied by the host environment.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Células da Medula Óssea
/
Transdução de Sinais
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Receptores da Neurocinina-1
Limite:
Animals
Idioma:
En
Ano de publicação:
2013
Tipo de documento:
Article