Emodin improves lipopolysaccharide-induced microcirculatory disturbance in rat mesentery.
Microcirculation
; 20(7): 617-28, 2013 Oct.
Article
em En
| MEDLINE
| ID: mdl-23551520
ABSTRACT
OBJECTIVE:
Sepsis is a systemic inflammatory response syndrome. Emodin is a major ingredient of Rheum Palmatum, a Chinese herb that is widely used in China for treatment of endotoxemia-related diseases. This study intended to examine the effect of Emodin on LPS-induced rat mesenteric microcirculatory disturbance and the underlying mechanisms.METHODS:
The male Wistar rats received LPS (5 mg/kg/hr) for 90 min, with or without administration of Emodin (10 mg/kg/hr) by enema 30 min before (pre-treatment) or after (post-treatment) LPS infusion, and the dynamics of mesenteric microcirculation were determined by inverted intravital microscopy. Expression of adhesion molecules and TLR4, NF-κB p65, ICAM-1, MPO, and AP-1 in mesentery tissue was evaluated by flow cytometry and Western-blot, respectively.RESULTS:
Pre or post-treatment with Emodin significantly ameliorated LPS-induced leukocyte emigration, reactive oxygen species production and albumin leakage, and the expression of TLR4, NF-κB p65, ICAM-1, MPO and AP-1 in mesentery.CONCLUSIONS:
These results demonstrate the beneficial role of Emodin in attenuating the LPS-induced microcirculatory disturbance, and support the use of Emodin for patients with endotoxemia.Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Fluxo Sanguíneo Regional
/
Lipopolissacarídeos
/
Emodina
/
Inibidores de Proteínas Quinases
/
Mesentério
/
Microcirculação
Limite:
Animals
/
Humans
/
Male
Idioma:
En
Ano de publicação:
2013
Tipo de documento:
Article