Regulation of glucocorticoid metabolism in the boar testis and caput epididymidis by the gonadotrophin-cAMP signalling pathway.
Cell Tissue Res
; 352(3): 751-60, 2013 Jun.
Article
em En
| MEDLINE
| ID: mdl-23568656
ABSTRACT
In target tissues, cortisol is metabolised by two 11ß-hydroxysteroid dehydrogenase (11ßHSD) isoenzymes, namely 11ßHSD1 and 11ßHSD2, both of which are co-expressed in the boar testis and reproductive tract. The present study has assessed whether cortisol-cortisone metabolism in boar testis and caput epididymidis can be regulated via the gonadotrophin-cAMP signalling pathway. 11ßHSD activities were measured by using a radiometric conversion assay in static tissue culture. In both testis and caput epididymidis, the net reduction of cortisone but not the net oxidation of cortisol, was significantly decreased by luteinising hormone (by 53 ± 20% and 45 ± 9%, respectively, P < 0.05), forskolin (by 60 ± 7% and 57 ± 9%, respectively, P < 0.01) and 8-bromo-cAMP (by 54 ± 4% and 64 ± 1%, respectively, P < 0.01). This suppression of 11-ketosteroid reductase activity in the boar testis by forskolin could be attenuated by the protein kinase A (PKA) inhibitor, H89. Hence, within the boar testis and the caput epididymidis, the local actions of glucocorticoids are modulated by gonadotrophin-cAMP-PKA signalling via their selective effects on the reductase activity of 11ßHSD.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Suínos
/
Testículo
/
Transdução de Sinais
/
AMP Cíclico
/
Epididimo
/
Glucocorticoides
/
Gonadotropinas
Limite:
Animals
/
Humans
/
Male
Idioma:
En
Ano de publicação:
2013
Tipo de documento:
Article