Basigin, a new, broadly distributed member of the immunoglobulin superfamily, has strong homology with both the immunoglobulin V domain and the beta-chain of major histocompatibility complex class II antigen.
J Biochem
; 107(2): 316-23, 1990 Feb.
Article
em En
| MEDLINE
| ID: mdl-2361961
ABSTRACT
Lotus tetragonolobus agglutinin (LTA) binds preferentially to early embryonic cells in the mouse. The affinity-purified antibody raised against LTA receptors from embryonal carcinoma cells were used to screen a lambda gt11 expression library of F9 embryonal carcinoma cells, resulting in detection of a cDNA clone specifying a new glycoprotein termed "basigin." The glycoprotein has been suggested to be a transmembrane one, and was found to be a new member of the immunoglobulin (Ig) superfamily. The molecular weight of basigin was largely in the range between 43,000 and 66,000, while that of the peptide portion with a putative signal sequence was inferred to be about 30,000. Significant levels of basigin mRNA were detected not only in embryonal carcinoma cells, but also in mouse embryos at 9-15 days of gestation and in various organs of the adult mouse. The Ig-like domain of basigin is unique, since it has strong homology to both the beta-chain of major histocompatibility class II antigen and the Ig V domain. The number of amino acids between the two conserved cysteine residues is intermediate between those of the Ig V and C domains. Therefore, basigin is an interesting protein in connection with the molecular evolution of the superfamily.
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Base de dados:
MEDLINE
Assunto principal:
Região Variável de Imunoglobulina
/
Genes de Imunoglobulinas
/
Glicoproteínas de Membrana
/
Proteínas Sanguíneas
/
Glicoproteínas
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Antígenos de Histocompatibilidade Classe II
/
Antígenos CD
/
Proteínas Aviárias
/
Antígenos de Neoplasias
/
Antígenos de Superfície
Limite:
Animals
/
Pregnancy
Idioma:
En
Ano de publicação:
1990
Tipo de documento:
Article