Topical application of the lectin Artin M accelerates wound healing in rat oral mucosa by enhancing TGF-ß and VEGF production.
Wound Repair Regen
; 21(3): 456-63, 2013.
Article
em En
| MEDLINE
| ID: mdl-23627356
ABSTRACT
The lectin Artin M has been shown to accelerate the wound-healing process. The aims of this study were to evaluate the effects of Artin M on wound healing in the palatal mucosa of rats and to investigate the effects of Artin M on transforming growth factor beta (TGF-ß) and vascular endothelial growth factor (VEGF) secretion by rat gingival fibroblasts. A surgical wound was created on the palatal mucosa of 72 rats divided into three groups according to treatment C--Control (nontreated), A--Artin M gel, and V--Vehicle. Eight animals per group were sacrificed at 3, 5, and 7 days postsurgery for histology, immunohistochemistry and determination of the levels of cytokines, and growth factors. Gingival fibroblasts were incubated with 2.5 µg/mL of Artin M for 24, 48, and 72 hours. The expression of VEGF and TGF-ß was determined by enzyme-linked immunosorbent assay. Histologically, at day 7, the Artin M group showed earlier reepithelialization, milder inflammatory infiltration, and increased collagen fiber formation, resulting in faster maturation of granular tissue than in the other groups (p < 0.05). Artin M-induced cell proliferation in vivo and promoted a greater expression of TGF-ß and VEGF in both experiments (p < 0.05). Artin M was effective in healing oral mucosa wounds in rats and was associated with increased TGF-ß and VEGF release, cell proliferation, reepithelialization, and collagen deposition and arrangement of fibers.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Cicatrização
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Ferimentos e Lesões
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Fator de Crescimento Transformador beta
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Fator A de Crescimento do Endotélio Vascular
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Lectinas
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Mucosa Bucal
Tipo de estudo:
Prognostic_studies
Limite:
Animals
Idioma:
En
Ano de publicação:
2013
Tipo de documento:
Article