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Tonsillar CD4+CD25+ regulatory T cells from IgA nephropathy patients have decreased immunosuppressive activity in experimental IgA nephropathy rats.
Huang, Hongdong; Peng, Youming; Long, Xi-Dai; Liu, Zhihua; Wen, Xiaojun; Jia, Meng; Liang, Yumei; Huang, Anlan.
Afiliação
  • Huang H; Division of Nephrology, Beijing Shijitan Hospital, Capital Medical University, Beijing, PR China. huanghd1126@126.com
Am J Nephrol ; 37(5): 472-80, 2013.
Article em En | MEDLINE | ID: mdl-23635548
BACKGROUND/AIM: CD4+CD25+ regulatory T (Treg) cells are of critical importance for maintenance of tolerance. We showed that the number of CD4+CD25+ Treg cells was significantly lower in tonsils of patients with IgA nephropathy (IgAN); however, the function of tonsillar CD4+CD25+ Treg cells in IgAN has not been reported. The aim of this study was to investigate the effect of tonsillar CD4+CD25+ Treg cells of IgAN patients on experimental IgAN in rats. METHODS: Tonsillar CD4+CD25+ Treg cells were isolated by magnetic beads. A total of 2 × 10(6) CD4+CD25+ Treg cells were transferred into rats that were previously orally immunized over a period of 14 weeks and subsequently received an injection of BSA into the tail vein on 3 consecutive days. Urine protein and erythrocytes were measured. Glomerular injury was assessed by histopathology. Plasminogen activator inhibitor type 1 (PAI-1), interleukin (IL)-6 and transforming growth factor (TGF)-ß1 in mesangial cells of rats were examined by reverse transcription PCR. Serum IgA and C3 and supernatants of IL-2, IL-4 and IL-6 in splenic cells were analysed by ELISA. Transferred tonsillar CD4+CD25+ Treg cells were tracked by reverse transcription PCR and flow cytometry. RESULTS: IgA deposition in the mesangial region and the glomerular planar area and the number of cells, levels of serum IgA and supernatant IL-2, IL-4 and IL-6 in splenic cells and PAI-1, IL-6 and TGF-ß1 expression in renal mesangial cells of rats that received CD4+CD25+ Treg cells from IgAN patients were significantly higher than in rats that received CD4+CD25+ Treg cells from the control group, although they were dramatically lower compared with rats treated without CD4+CD25+ Treg cells. Transferred tonsillar CD4+CD25+ Treg cells migrated predominantly to secondary lymphoid organs but not to the kidneys. CONCLUSION: Dysfunction of tonsillar CD4+CD25+ Treg cells may be an important cause of IgAN progression.
Assuntos

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Tonsila Palatina / Linfócitos T Reguladores / Glomerulonefrite por IGA / Tolerância Imunológica Tipo de estudo: Prognostic_studies Limite: Adolescent / Adult / Animals / Female / Humans / Male Idioma: En Ano de publicação: 2013 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Tonsila Palatina / Linfócitos T Reguladores / Glomerulonefrite por IGA / Tolerância Imunológica Tipo de estudo: Prognostic_studies Limite: Adolescent / Adult / Animals / Female / Humans / Male Idioma: En Ano de publicação: 2013 Tipo de documento: Article