Inhibition of lipopolysaccharide-induced iNOS and COX-2 expression by indole alkaloid, 3-(hydroxymethyl)-6,7-dihydroindolo[2,3-a]quinolizin-(12H)-one, via NF-κB inactivation in RAW 264.7 macrophages.
Planta Med
; 79(9): 782-7, 2013 Jun.
Article
em En
| MEDLINE
| ID: mdl-23670629
ABSTRACT
3-(Hydroxymethyl)-6,7-dihydroindolo[2,3-a]quinolizin-(12H)-one is a bioactive indole alkaloid isolated from Nauclea officinalis, a plant species which is used as a traditional Chinese medicine. We investigated the anti-inflammatory properties of 3-(hydroxymethyl)-6,7-dihydroindolo[2,3-a]quinolizin-(12H)-one in RAW 264.7 murine macrophages. The results indicated that it inhibited the overproduction of NO and the release of TNF-α. Furthermore, this compound inhibited the expression of iNOS and COX-2 proteins, the enzymatic activity of iNOS, and the translocation of NF-κB to the nucleus induced by LPS. Therefore, we suggested that the effect of 3-(hydroxymethyl)-6,7-dihydroindolo[2,3-a]quinolizin-(12H)-one-mediated inhibition of the expression of LPS-induced iNOS and COX-2 genes is due to the suppression of NF-κB activation in the transcriptional level.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Quinolizinas
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Anti-Inflamatórios não Esteroides
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Lipopolissacarídeos
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NF-kappa B
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Alcaloides Indólicos
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Óxido Nítrico Sintase Tipo II
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Inibidores de Ciclo-Oxigenase 2
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Indóis
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Macrófagos
Limite:
Animals
Idioma:
En
Ano de publicação:
2013
Tipo de documento:
Article