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Nanotopographic surfaces with defined surface chemistries from amyloid fibril networks can control cell attachment.
Reynolds, Nicholas P; Styan, Katie E; Easton, Christopher D; Li, Yali; Waddington, Lynne; Lara, Cecile; Forsythe, John S; Mezzenga, Raffaele; Hartley, Patrick G; Muir, Benjamin W.
Afiliação
  • Reynolds NP; Materials Science and Engineering, CSIRO, Private Bag 10, Bayview Avenue, Clayton, Vic 3169, Australia. nick.reynolds@csiro.au
Biomacromolecules ; 14(7): 2305-16, 2013 Jul 08.
Article em En | MEDLINE | ID: mdl-23701412
We show for the first time the possibility of using networks of amyloid fibrils, adsorbed to solid supports and with plasma polymer coatings, for the fabrication of chemically homogeneous surfaces with well-defined nanoscale surface features reminiscent of the topography of the extracellular matrix. The robust nature of the fibrils allows them to withstand the plasma polymer deposition conditions used with no obvious deleterious effect, thus enabling the underlying fibril topography to be replicated at the polymer surface. This effect was seen despite the polymer coating thickness being an order of magnitude greater than the fibril network. The in vitro culture of fibroblast cells on these surfaces resulted in increased attachment and spreading compared to flat plasma polymer films with the same chemical composition. The demonstrated technique allows for the rapid and reproducible fabrication of substrates with nanoscale fibrous topography that we believe will have applications in the development of new biomaterials allowing, for example, the investigation of the effect of extracellular matrix mimicking nanoscale morphology on cellular phenotype.
Assuntos

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Materiais Biocompatíveis / Nanoestruturas / Amiloide Limite: Animals Idioma: En Ano de publicação: 2013 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Materiais Biocompatíveis / Nanoestruturas / Amiloide Limite: Animals Idioma: En Ano de publicação: 2013 Tipo de documento: Article