Determination of levamisole in the plasma of patients with falciparum malaria using high-performance liquid chromatography.
Clin Lab
; 59(3-4): 439-44, 2013.
Article
em En
| MEDLINE
| ID: mdl-23724638
ABSTRACT
BACKGROUND:
The oral antihelmintic drug levamisole reduces sequestration of late stage parasites in falciparum malaria. Levamisole has been also identified as a cocaine adulterant. In the present study, authors developed a sensitive and selective HPLC-assay for the determination of levamisole in the plasma from patients with falciparum malaria.METHODS:
Chromatographic separation was achieved by using a C18 column and with an isocratic elution system comprising phosphate buffer and acetonitrile. The eluate was monitored at 235 nm by diode array detection.RESULTS:
The calibration curve for levamisole was linear in the range from 50 to 2000 ng/mL (r2 > 0.999). The limit of quantification was 28 ng/mL and the inter- and intraday coefficients of variation were less than 7%. No interference from commonly prescribed malaria treatments was observed.CONCLUSIONS:
The HPLC method is simple, rapid, and robust and is suited for monitoring levamisole patients in routine or toxicological studies.
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Base de dados:
MEDLINE
Assunto principal:
Levamisol
/
Cromatografia Líquida de Alta Pressão
/
Malária Falciparum
Tipo de estudo:
Prognostic_studies
Limite:
Humans
Idioma:
En
Ano de publicação:
2013
Tipo de documento:
Article