Does morphology of carotid plaque depend on patient's oxidative stress?
Clin Biochem
; 46(12): 1030-1035, 2013 Aug.
Article
em En
| MEDLINE
| ID: mdl-23726810
ABSTRACT
OBJECTIVES:
This study explored the relationship between oxidative stress biomarkers and stability of carotid plaque. We decided to analyze the broad range of parameters describing oxidative stress in patients with carotid stenosis. DESIGN ANDMETHODS:
124 consecutive patients undergoing carotid endarterectomy were enrolled in the study group. The control group consisted of 49 patients without symptoms of atherosclerosis. The stability of carotid plaques was assessed using GSM (gray-scale median) scoring system and the study group was divided into three subgroups according to echogenicity of the plaque. The following parameters of oxidative stress/DNA damage were analyzed i) urinary excretion of the products of oxidative DNA damage repair; ii) the background level of 8-oxo-7,8-dihydro-2'-deoxyguanosine in leukocytes' DNA and in atherosclerotic plaques; and iii) the concentrations of antioxidant vitamins, uric acid and C-reactive protein in plasma.RESULTS:
Oxidative stress (described by redox status) was higher in the patient group than in the control group. There is a correlation between oxidative stress of the patients and stability of the plaque, echolucent plaques (GSM<25) being associated with the highest antioxidant level and lowest excretion of DNA repair markers.CONCLUSIONS:
The plaque formation/morphology may depend on local environment and is independent of oxidative stress/inflammation observed on the level of the whole body.Palavras-chave
8-oxo-7,8-dihydro-2'-deoxyguanosine; 8-oxo-7,8-dihydroguanine; 8-oxoGua; 8-oxodG; Atherosclerosis; C-reactive protein; CRP; Carotid stenosis; DNA damage; GC/MS; GSM; HPLC; IQR; Oxidative stress; ROS; gas chromatography coupled with mass spectrometry detection; gray-scale median scoring system; high-performance liquid chromatography; interquartile range; reactive oxygen species
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Estenose das Carótidas
/
Estresse Oxidativo
Tipo de estudo:
Observational_studies
/
Risk_factors_studies
Limite:
Aged
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Female
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Humans
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Male
/
Middle aged
Idioma:
En
Ano de publicação:
2013
Tipo de documento:
Article