Methanol extract of Evodia lepta displays Syk/Src-targeted anti-inflammatory activity.
J Ethnopharmacol
; 148(3): 999-1007, 2013 Jul 30.
Article
em En
| MEDLINE
| ID: mdl-23747536
ABSTRACT
ETHNOPHARMACOLOGICAL RELEVANCE Evodia lepta (Spreng.) Merr., in the Rutaceae family, is a medicinal plant traditionally used to treat inflammatory symptoms such as in meningitis and hepatitis. However, no study has systematically investigated its anti-inflammatory activities including its molecular mechanism. MATERIALS AND METHODS:
The effects of a methanol extract from the roots Evodia lepta (El-ME) were evaluated using lipopolysaccharide (LPS)-treated RAW264.7 cells producing nitric oxide (NO) and prostaglandin E2 (PGE2), and an HCl/ethanol-induced mouse gastritis model. Target molecules were identified by analyzing the activation of transcription factors and their upstream kinases.RESULTS:
El-ME reduced the production of NO and PGE2 from LPS-activated RAW264.7 cells in a dose-dependent manner. El-ME also ameliorated the gastritis symptoms of EtOH/HCl-treated mice. The extract suppressed production of mRNA for the inducible NO synthase (iNOS) and cyclooxygenase (COX)-2; the nuclear translocation of nuclear factor (NF)-κB; the phosphorylation of upstream kinases that activate NF-κB; and the kinase activities of Syk and Src.CONCLUSION:
The anti-inflammatory effects of El-ME might be due to its suppression of Syk/Src and NF-κB. Considering the in vitro and in vivo efficacy of El-ME, Evodia lepta could be developed into an anti-inflammatory herbal remedy.Palavras-chave
(TNF)-α; 3-4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide; Anti-inflammatory; CMC; COX; EIA; ELISA; Evodia lepta (Spreng.) Merr.; IκB kinase; IκBκ; Iκκ; LPS; MTT; NF-κB; NO; PG; PI3K; RT-PCR; Rutaceae; Src; Syk; TLR; Toll-like receptors (TLR); cyclooxygenase; enzyme immunoassay; enzyme-linked immunosorbent assay; iNOS; inducible NO synthase; inhibitor of kappa B alpha; lipopolysaccharide; nitric oxide; nuclear factor-κB; phosphoinositide 3-kinase; prostaglandin; reverse transcriptase-polymerase chain reaction; sodium carboxymethylcellulose; spleen tyrosine kinase; tumor necrosis factor
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Extratos Vegetais
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Evodia
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Anti-Inflamatórios
Tipo de estudo:
Prognostic_studies
Limite:
Animals
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Humans
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Male
Idioma:
En
Ano de publicação:
2013
Tipo de documento:
Article