Dynamic microtubules produce an asymmetric E-cadherin-Bazooka complex to maintain segment boundaries.
J Cell Biol
; 201(6): 887-901, 2013 Jun 10.
Article
em En
| MEDLINE
| ID: mdl-23751496
ABSTRACT
Distributing junctional components around the cell periphery is key for epithelial tissue morphogenesis and homeostasis. We discovered that positioning of dynamic microtubules controls the asymmetric accumulation of E-cadherin. Microtubules are oriented preferentially along the dorso-ventral axis in Drosophila melanogaster embryonic epidermal cells, and thus more frequently contact E-cadherin at dorso-ventral cell-cell borders. This inhibits RhoGEF2, reducing membrane recruitment of Rho-kinase, and increasing a specific E-cadherin pool that is mobile when assayed by fluorescence recovery after photobleaching. This mobile E-cadherin is complexed with Bazooka/Par-3, which in turn is required for normal levels of mobile E-cadherin. Mobile E-cadherin-Bazooka prevents formation of multicellular rosette structures and cell motility across the segment border in Drosophila embryos. Altogether, the combined action of dynamic microtubules and Rho signaling determines the level and asymmetric distribution of a mobile E-cadherin-Bazooka complex, which regulates cell behavior during the generation of a patterned epithelium.
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Base de dados:
MEDLINE
Assunto principal:
Caderinas
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Proteínas de Drosophila
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Peptídeos e Proteínas de Sinalização Intracelular
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Drosophila melanogaster
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Microtúbulos
Limite:
Animals
Idioma:
En
Ano de publicação:
2013
Tipo de documento:
Article