Genome-wide profiling of human cap-independent translation-enhancing elements.
Nat Methods
; 10(8): 747-50, 2013 Aug.
Article
em En
| MEDLINE
| ID: mdl-23770754
ABSTRACT
We report an in vitro selection strategy to identify RNA sequences that mediate cap-independent initiation of translation. This method entails mRNA display of trillions of genomic fragments, selection for initiation of translation and high-throughput deep sequencing. We identified >12,000 translation-enhancing elements (TEEs) in the human genome, generated a high-resolution map of human TEE-bearing regions (TBRs), and validated the function of a subset of sequences in vitro and in cultured cells.
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Base de dados:
MEDLINE
Assunto principal:
Iniciação Traducional da Cadeia Peptídica
/
RNA Mensageiro
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Genoma Humano
Limite:
Humans
Idioma:
En
Ano de publicação:
2013
Tipo de documento:
Article