Accelerated solvent extraction and pH-zone-refining counter-current chromatographic purification of yunaconitine and 8-deacetylyunaconitine from Aconitum vilmorinianum Kom.
J Sep Sci
; 36(16): 2680-5, 2013 Aug.
Article
em En
| MEDLINE
| ID: mdl-23784883
ABSTRACT
This study aimed to seek an efficient method to extract and purify yunaconitine and 8-deacetylyunaconitine from Aconitum vilmorinianum Kom. by accelerated solvent extraction combined with pH-zone-refining counter-current chromatography. The major extraction parameters for accelerated solvent extraction were optimized by an orthogonal test design L9 (3)(4). Then a separation and purification method was established using pH-zone-refining counter-current chromatography with a two-phase solvent system composed of petroleum ether/ethyl acetate/methanol/water (5528, v/v) with 10 mM triethylamine in the upper phase and 10 mM HCl in the lower phase. From 2 g crude extract, 224 mg of 8-deacetylyunaconitine (I) and 841 mg of yunaconitine (II) were obtained with a purity of over 98.0%. The chemical structures were identified by ESI-MS and (1)H and (13)C NMR spectroscopy.
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Base de dados:
MEDLINE
Assunto principal:
Aconitina
/
Extratos Vegetais
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Distribuição Contracorrente
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Aconitum
Tipo de estudo:
Evaluation_studies
Idioma:
En
Ano de publicação:
2013
Tipo de documento:
Article