Isolation and characterization of human breast cancer cells with SOX2 promoter activity.
Biochem Biophys Res Commun
; 437(2): 205-11, 2013 Jul 26.
Article
em En
| MEDLINE
| ID: mdl-23796710
ABSTRACT
Sex determining region Y-box 2 (SOX2) is well known as one of the "stemness" factors and is often expressed in cancers including breast cancer. In this study, we developed a reporter system using fluorescent protein driven by the promoter for SOX2 gene to detect and isolate living SOX2-positive cells. Using this system, we determined that SOX2 promoter activities were well correlated with SOX2 mRNA expression levels in 5 breast cancer cell lines, and that the cell population with positive SOX2 promoter activity (pSp-T(+)) isolated from one of the 5 cell lines, MCF-7 cells, showed a high SOX2 protein expression and high sphere-forming activity compared with very low promoter activity (pSp-T(low/-)). The pSp-T(+) population expressed higher mRNA levels of several stemness-related genes such as CD44, ABCB1, NANOG and TWIST1 than the pSp-T(low/-) population whereas the two populations expressed CD24 at similar levels. These results suggest that the cell population with SOX2 promoter activity contains cancer stem cell (CSC)-like cells which show different expression profiles from those of CSC-marker genes previously recognized in human breast cancers.
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Base de dados:
MEDLINE
Assunto principal:
Neoplasias da Mama
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Regiões Promotoras Genéticas
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Fatores de Transcrição SOXB1
Limite:
Female
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Humans
Idioma:
En
Ano de publicação:
2013
Tipo de documento:
Article