A mass-differentiated library strategy for identification of sugar nucleotidyltransferase activities from cell lysates.
Anal Biochem
; 441(1): 8-12, 2013 Oct 01.
Article
em En
| MEDLINE
| ID: mdl-23811154
ABSTRACT
Sugar nucleotidyltransferases, or nucleotide sugar pyrophosphorylases, are ubiquitous enzymes whose activities have been correlated to disease states and pathogen virulence. Here we report a rapid "one-pot" method to identify a range of sugar nucleotidyltransferase activities of purified proteins or in cell lysates using a mass-differentiated carbohydrate library designed for mass spectrometry-based analysis.
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Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Escherichia coli
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Metabolismo dos Carboidratos
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Bibliotecas de Moléculas Pequenas
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Nucleotidiltransferases
Tipo de estudo:
Diagnostic_studies
Idioma:
En
Ano de publicação:
2013
Tipo de documento:
Article