Visualization of immune cell infiltration in experimental viral myocarditis by (19)F MRI in vivo.
MAGMA
; 27(1): 101-6, 2014 Feb.
Article
em En
| MEDLINE
| ID: mdl-23824166
ABSTRACT
OBJECTIVE:
This paper introduces a new approach permitting for the first time a specific, non-invasive diagnosis of myocarditis by visualizing the infiltration of immune cells into the myocardium. MATERIALS ANDMETHODS:
The feasibility of this approach is shown in a murine model of viral myocarditis. Our study uses biochemically inert perfluorocarbons (PFCs) known to be taken up by circulating monocytes/macrophages after intravenous injection.RESULTS:
In vivo (19)F MRI at 9.4 T demonstrated that PFC-loaded immune cells infiltrate into inflamed myocardial areas. Because of the lack of any fluorine background in the body, detected (19)F signals of PFCs are highly specific as confirmed ex vivo by flow cytometry and histology.CONCLUSION:
Since PFCs are a family of compounds previously used clinically as blood substitutes, the technique described in our paper holds the potential as a new imaging modality for the diagnosis of myocarditis in man.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Imageamento por Ressonância Magnética
/
Miocardite
Limite:
Animals
Idioma:
En
Ano de publicação:
2014
Tipo de documento:
Article