Spatial-spectral coupling in coherent anti-Stokes Raman scattering microscopy.
Opt Express
; 21(13): 15298-307, 2013 Jul 01.
Article
em En
| MEDLINE
| ID: mdl-23842316
ABSTRACT
Coherent anti-Stokes Raman scattering (CARS) microscopy is a third-order nonlinear optical technique which permits label-free, molecule-specific hyperspectral imaging. The interference between coherent resonant and non-resonant terms leads to well known distortions in the vibrational spectrum, requiring the use of retrieval algorithms. It also leads to spatial imaging distortions, largely due to the Gouy phase, when objects are smaller than the Rayleigh range. Here we consider that the focal position and spectral contributions to the nonlinear image formation are intrinsically coupled and cannot be corrected by conventional retrieval methods.
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MEDLINE
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En
Ano de publicação:
2013
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Article