Modulation of stress related protein genes in the bass (Epinephelus guaza) caught from the Gulf of Suez, the Red Sea, Egypt.

ABSTRACT

Impact of chemical pollution on expression of stress protein genes in the bass Epinephelus guaga collected from several locations including Suez Oil Production Port (Floating port), Atakah Fishing Port, Adabiya Port and Tawfik Port in Suez Governorate, Egypt, was investigated. In the current study, levels of polycyclic aromatic hydrocarbons (PAHs) in water and fish samples collected from Suez Gulf were assessed. In addition, gills and liver tissues of caught bass fish were used to address the interaction between pollution status and the expression of stress-related genes (Hsp70a, Hsp70b, Hsp47, MT and CYP1A). Our analysis demonstrated that levels of PAHs in Floating and Tawfik ports were higher than those found in the Atakah Fishing Port and the Adabiya Port. In addition, MDA and PC contents were significantly higher in gills and liver tissues collected from Floating and Tawfik ports than those collected from Adabiya and Atakah ports. In correlation to the above results, all fish collected from the Floating and Tawfik ports presented a significant increase in Hsp-, MT- and CYP1A-mRNAs. On the other hand, fish samples collected from the Atakah Fishing and Adabiya ports showed no induction of the stress-related genes transcription in such tissues. In conclusion, the current research demonstrates that remarkable increase in PAH contaminants levels in Floating and Tawfik ports are correlated with the levels stress protein-related genes transcription in E. guaga gills and liver tissues.