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Basic helix-loop-helix transcription factors JASMONATE-ASSOCIATED MYC2-LIKE1 (JAM1), JAM2, and JAM3 are negative regulators of jasmonate responses in Arabidopsis.
Sasaki-Sekimoto, Yuko; Jikumaru, Yusuke; Obayashi, Takeshi; Saito, Hikaru; Masuda, Shinji; Kamiya, Yuji; Ohta, Hiroyuki; Shirasu, Ken.
Afiliação
  • Sasaki-Sekimoto Y; RIKEN Plant Science Center, Tsurumi-ku, Yokohama, Kanagawa 230-0045, Japan.
Plant Physiol ; 163(1): 291-304, 2013 Sep.
Article em En | MEDLINE | ID: mdl-23852442
ABSTRACT
Jasmonates regulate transcriptional reprogramming during growth, development, and defense responses. Jasmonoyl-isoleucine, an amino acid conjugate of jasmonic acid (JA), is perceived by the protein complex composed of the F-box protein CORONATINE INSENSITIVE1 (COI1) and JASMONATE ZIM DOMAIN (JAZ) proteins, leading to the ubiquitin-dependent degradation of JAZ proteins. This activates basic helix-loop-helix-type MYC transcription factors to regulate JA-responsive genes. Here, we show that the expression of genes encoding other basic helix-loop-helix transcription factors, JASMONATE ASSOCIATED MYC2-LIKE1 (JAM1), JAM2, and JAM3, is positively regulated in a COI1- and MYC2-dependent manner in Arabidopsis (Arabidopsis thaliana). However, contrary to myc2, the jam1jam2jam3 triple mutant exhibited shorter roots when treated with methyl jasmonate (MJ), indicating enhanced responsiveness to JA. Our genome-wide expression analyses revealed that key jasmonate metabolic genes as well as a set of genes encoding transcription factors that regulate the JA-responsive metabolic genes are negatively regulated by JAMs after MJ treatment. Consistently, loss of JAM genes resulted in higher accumulation of anthocyanin in MJ-treated plants as well as higher accumulation of JA and 12-hydroxyjasmonic acid in wounded plants. These results show that JAMs negatively regulate the JA responses in a manner that is mostly antagonistic to MYC2.
Assuntos

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Arabidopsis / Ciclopentanos / Proteínas de Arabidopsis / Fatores de Transcrição Hélice-Alça-Hélice Básicos / Oxilipinas Tipo de estudo: Risk_factors_studies Idioma: En Ano de publicação: 2013 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Arabidopsis / Ciclopentanos / Proteínas de Arabidopsis / Fatores de Transcrição Hélice-Alça-Hélice Básicos / Oxilipinas Tipo de estudo: Risk_factors_studies Idioma: En Ano de publicação: 2013 Tipo de documento: Article