Testing two models describing how methylome-wide studies in blood are informative for psychiatric conditions.
Epigenomics
; 5(4): 367-77, 2013 Aug.
Article
em En
| MEDLINE
| ID: mdl-23895651
ABSTRACT
AIM:
As the primary relevant tissue (brain) for psychiatric disorders is commonly not available, we aimed to investigate whether blood can be used as a proxy in methylation studies on the basis of two models. In the 'signature' model methylation-disease associations occur because a disease-causing factor affected methylation in the blood. In the 'mirror-site' model the methylation status in the blood is correlated with the corresponding disease-causing site in the brain. MATERIALS, METHODS &RESULTS:
Methyl-binding domain enrichment and next-generation sequencing of the blood, cortex and hippocampus from four haloperidol-treated and ten untreated C57BL/6 mice revealed high levels of correlation in methylation across tissues. Despite the treatment inducing a large number of methylation changes, this correlation remains high.CONCLUSION:
Our results show that, consistent with the signature model, factors that affect brain processes (i.e., haloperidol) leave biomarker signatures in the blood and, consistent with the mirror-site model, the methylation status of many sites in the blood mirror those in the brain.
Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Encéfalo
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Biomarcadores
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Metilação de DNA
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Proteínas de Ligação a DNA
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Modelos Biológicos
Tipo de estudo:
Diagnostic_studies
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Prognostic_studies
Limite:
Animals
Idioma:
En
Ano de publicação:
2013
Tipo de documento:
Article