[Determination of unknown impurities in cefotiam hexetil by HPLC-MS/MS].
Sichuan Da Xue Xue Bao Yi Xue Ban
; 44(3): 481-4, 493, 2013 May.
Article
em Zh
| MEDLINE
| ID: mdl-23898540
ABSTRACT
OBJECTIVE:
To detect unknown impurities in raw drug material of cefotiam hexetil.METHODS:
High performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) was employed for the determination of impurities in cefotiam hexetil. Agilent SB-C18 column (150 mm x 2.1 mm i. d. , 3.5 microm particles) was used for chromatographic separations of cofotiam hexetil dissolved in deionized water, with mobile phase consisting of (A) 0.1% formic acid and (B) acetonitrile and timed gradient program T (min)/B (%) 0/3, 5/3, 15/20, 20/40, 30/60, 40/80. The flow rate was set at 0. 3 mL/min with DAD detector wavelength fixed at 254 nm. Electrospray ionization source was applied and operated in positive ion MRM mode. The source voltage was kept at 4 kV and cone voltage was 100 V with the mass range m/z 50-1000. Nitrogen was used as nebulizing gas and the nebulizer pressure was 40 psi. The drying gas temperature was 350 degrees C and the drying gas flow was 10 L/min. Results Unknown impurities of cefotiam hexetil were identified. Substance 1 was delta3-isomer of cefotiam hexetil. The structures of 3 other substances were also determined.CONCLUSION:
The method is sensitive, rapid and credible for the analysis of cefotiam hexetil and its related impurities, which can be applied in quality control of cefotiam hexetil.
Buscar no Google
Base de dados:
MEDLINE
Assunto principal:
Cefotiam
/
Contaminação de Medicamentos
/
Cromatografia Líquida de Alta Pressão
/
Espectrometria de Massas em Tandem
Idioma:
Zh
Ano de publicação:
2013
Tipo de documento:
Article