CD11b(+) cells in donor-specific transfusion prolonged allogenic skin graft survival through indoleamine 2,3-dioxygenase.
Cell Immunol
; 283(1-2): 81-90, 2013.
Article
em En
| MEDLINE
| ID: mdl-23933136
The aim of this study is to show the effect of donor-specific transfusion (DST) in inducing immunological tolerance mediated by regulatory T cells (Treg) and indoleamine 2,3-dioxygenase (IDO). Skin grafts from H2(d) Balb/c were transplanted into H2(k) C3H/He 7days after the infusion of donor splenocytes, isolated each immune cell populations. Graft survival prolonged in recipients who received splenocytes, MHC class II(+) CD90(-) cells and CD3(-)CD19(-) cells (p<0.001, p<0.05 and p<0.01, respectively). CD11b(+) cell infusion resulted in prolongation of graft survival when compared to CD11c(+) cell infusion (p<0.01). Foxp3(+)CD4(+)CD25(+) T cells were increased after the transplant in recipients infused with CD11b(+) cells (p<0.05). The mixed lymphocyte reaction showed donor-specificity (p<0.001). High IDO expression was observed in CD11b(+) cell infusion group. Graft survival with DST using IDO antagonist (1MT) were not prolonged. In conclusion, DST allows induction of donor-specific tolerance which involves Foxp3(+)CD4(+)CD25(+) T cells and IDO expression.
Palavras-chave
5-bromo-2'-deoxyuridine; APC; BrdU; DC; DST; Donor-specific transfusion; IDO; IL; INF; Immunological tolerance; Indoleamine 2,3-doxygenase; MACS; MLR; NK; Regulatory T cell; antigen-presenting cells; dendritic cell; donor-specific transfusion; indoleamine 2,3-dioxygenase; interferon; interleukin; magnetic cell sorting; mixed lymphocyte reaction; natural killer
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Transplante de Pele
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Indolamina-Pirrol 2,3,-Dioxigenase
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Sobrevivência de Enxerto
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Tolerância Imunológica
Limite:
Animals
Idioma:
En
Ano de publicação:
2013
Tipo de documento:
Article