A proteorhodopsin-based biohybrid light-powering pH sensor.
Phys Chem Chem Phys
; 15(38): 15821-4, 2013 Oct 14.
Article
em En
| MEDLINE
| ID: mdl-23970242
ABSTRACT
The biohybrid sensor is an emerging technique for multi-functional detection that utilizes the instinctive responses or interactions of biomolecules. We develop a biohybrid pH sensor by taking advantage of the pH-dependent photoelectric characteristics of proteorhodopsin (pR). The transient absorption kinetics study indicates that the photoelectric behavior of pR is attributed to the varying lifetime of the M intermediate at different environmental pH values. This pR-based biohybrid light-powering sensor with microfluidic design can achieve real-time pH detection with quick response and high sensitivity. The results of this work would shed light on pR and its potential applications.
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Base de dados:
MEDLINE
Assunto principal:
Rodopsina
Idioma:
En
Ano de publicação:
2013
Tipo de documento:
Article