Prospective comparison of the detection rates of human enterovirus and parechovirus RT-qPCR and viral culture in different pediatric specimens.
J Clin Virol
; 58(2): 449-54, 2013 Oct.
Article
em En
| MEDLINE
| ID: mdl-23973350
BACKGROUND: Reverse-transcriptase quantitative real-time polymerase chain reaction (RT-qPCR) has become the gold standard for the diagnosis of human enterovirus (EV) and parechovirus (HPeV) infections. The detection rate of RT-qPCR in different pediatric body specimens has not been compared prospectively in a multicentre study. OBJECTIVES: This study compared the diagnostic detection rates of EV and HPeV RT-qPCR and viral culture in different specimens (feces, nasopharynx, blood, urine and cerebrospinal fluid (CSF)) of pediatric patients. STUDY DESIGN: This prospective, multicenter study performed an EV and HPeV RT-qPCR on nasopharynx, blood, urine, feces and CSF specimens and a viral culture on nasopharynx, feces and CSF specimens in symptomatic children<16 years. RESULTS: Of 285 included children EV was detected in 140 (49%) and HPeV in 44 (15%) children. Both EV and HPeV RT-qPCR had a higher sensitivity and negative predictive value than EV and HPeV viral culture, respectively. EV and HPeV RT-qPCR in feces specimen had the highest sensitivity (99.2% and 95.1%) of all specimens. Pooling results of specimens increased the detection rate for both viruses. CONCLUSION: Of all specimens, RT-qPCR in feces had the highest detection rate for both EV and HPeV in symptomatic pediatric patients. An EV was detected in all EV positive patients if a RT-qPCR was performed on both feces and CSF specimens or in both feces and urine specimens. HPeV was detected in all HPeV positive patients if a RT-qPCR was performed on both feces and CSF specimens, feces and nasopharynx specimens or CSF and nasopharynx specimens.
Palavras-chave
CSF; EV; Enterovirus; HEp-2; HPeV; Human parechovirus; NPV; PPV; Pediatric; RNA; RT-qPCR; Viral culture; cerebrospinal fluid; cynomolgus monkey kidney; human epidermoid cancer; human non-polio enterovirus; human parechovirus; negative predictive value; positive predictive value; reverse-transcriptase real time quantitative polymerase chain reaction; ribonucleic acid; tMK
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Enterovirus
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Infecções por Picornaviridae
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Reação em Cadeia da Polimerase Via Transcriptase Reversa
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Parechovirus
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Técnicas de Diagnóstico Molecular
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Infecções por Enterovirus
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Reação em Cadeia da Polimerase em Tempo Real
Tipo de estudo:
Clinical_trials
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Diagnostic_studies
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Observational_studies
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Prognostic_studies
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Risk_factors_studies
Limite:
Adolescent
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Child
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Child, preschool
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Female
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Humans
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Infant
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Male
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Newborn
Idioma:
En
Ano de publicação:
2013
Tipo de documento:
Article