Comparison of activation ability between feline and bovine oocytes.
Acta Vet Hung
; 61(4): 491-4, 2013 Dec.
Article
em En
| MEDLINE
| ID: mdl-23974933
ABSTRACT
Research comparing the activation sensitivity of oocytes to chemical treatment among mammalian species remains limited. We compared the activation ability of oocytes from bovine and feline ovaries when treated with ethanol alone, with ethanol and cycloheximide, and without any chemical treatment; the oocytes were then cultured for 72 h. After in vitro maturation (IVM), 5% of feline oocytes were activated and 1% were cleaved, whereas there were no prematurely activated bovine oocytes. Activation rates with ethanol and ethanol/cycloheximide were significantly higher (P < 0.01) in bovine oocytes than in feline oocytes (74.2% vs. 34.1% and 86.3% vs. 52.5%, respectively). Thus, our findings indicate that feline oocytes can be prematurely activated by the end of IVM, and that bovine oocytes may have a higher sensitivity of parthenogenetic activation to chemical treatment than do feline oocytes.
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MEDLINE
Assunto principal:
Oócitos
/
Partenogênese
Limite:
Animals
Idioma:
En
Ano de publicação:
2013
Tipo de documento:
Article