Experimental and theoretical analysis for improved microscope design of optical projection tomographic microscopy.
Opt Lett
; 38(17): 3398-401, 2013 Sep 01.
Article
em En
| MEDLINE
| ID: mdl-23988968
ABSTRACT
We present theoretical and experimental results of axial displacement of objects relative to a fixed condenser focal plane (FP) in optical projection tomographic microscopy (OPTM). OPTM produces three-dimensional, reconstructed images of single cells from two-dimensional projections. The cell rotates in a microcapillary to acquire projections from different perspectives where the objective FP is scanned through the cell while the condenser FP remains fixed at the center of the microcapillary. This work uses a combination of experimental and theoretical methods to improve the OPTM instrument design.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Tomografia Óptica
/
Microscopia
Idioma:
En
Ano de publicação:
2013
Tipo de documento:
Article