Preparation of fragment libraries for next-generation sequencing on the applied biosystems SOLiD platform.
Methods Enzymol
; 529: 185-200, 2013.
Article
em En
| MEDLINE
| ID: mdl-24011046
ABSTRACT
The primary purpose of this protocol is to prepare genomic DNA libraries that can then be analyzed by massively parallel next-generation sequencing on the Applied Biosystems SOLiD platform. This protocol can be adapted to next-generation sequencing workflows to ultimately generate up to 1 billion 50 bp sequence tags from the ends of each of the DNA molecules in the library in a single next-generation sequencing run.
Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
DNA
/
Biblioteca Gênica
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Sequenciamento de Nucleotídeos em Larga Escala
Idioma:
En
Ano de publicação:
2013
Tipo de documento:
Article