Anticorpos Monoclonais/química; Hepacivirus/metabolismo; Anticorpos de Cadeia Única/química; Proteínas Virais/imunologia; Anticorpos Monoclonais/metabolismo; Biologia Computacional; Escherichia coli; Anticorpos de Cadeia Única/metabolismo; Proteínas Virais/metabolismo

3-(1-pyridino)-1-propanesulfonates; Anti-HCV therapy; Antibody engineering; CDR; DAAs; E1E2; ELISA; FR; GT; HCV; HCVsp; HRP; Hepatitis C virus; IMAC; IPTG; IgG; LB medium; Leibovitz medium; MALLS; NDSB; NR; PCR; PEG-IFN; PVDF; R; RBV; SDS­PAGE; SOC; Single chain Fv fragment; TBS; Tris buffer saline; V(H); V(L); complementarity-determining region; direct-acting antivirals; enzyme-linked immunoabsorbent assay; framework region; genotype; heavy chain variable region; hepatitis C virus; horseradish peroxidase; immobilized metal affinity chromatography; immunoglobulin G; isopropylthio-ß-galactoside; light chain variable region; mAb; monoclonal antibody; multi-angle laser light scattering; non-reducing; pegylated interferon-α; polymerase chain reaction; polyvinylidene difluoride; reducing; ribavirin; scFv; serum-derived HCV particles; single chain antibody fragment; sodium dodecylsulfate­polyacrylamide gel electrophoresis; standard of care