Cloning and characterization of a 2-Cys peroxiredoxin from Babesia gibsoni.
J Vet Med Sci
; 76(1): 139-43, 2014 Jan.
Article
em En
| MEDLINE
| ID: mdl-24025459
ABSTRACT
Peroxiredoxins (Prxs) are a family of antioxidant enzymes. Here, we cloned a 2-Cys Prx, BgTPx-1, from the canine Babesia parasite B. gibsoni. Sequence identity between BgTPx-1 and 2-Cys Prx of B. bovis was 81% at the amino acid level. Enzyme activity assay by using recombinant BgTPx-1 (rBgTPx-1) indicated that BgTPx-1 has antioxidant activity. Antiserum from a mouse immunized with rBgTPx-1 reacted with parasite lysates and detect a protein with a monomeric size of 22 kDa and also a 44 kDa protein, which might be an inefficiently reduced dimer. BgTPx-1 was expressed in the cytoplasm of B. gibsoni merozoites. These results suggest that the BgTPx-1 may play a role to control redox balance in the cytoplasm of B. gibsoni.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Babesia
/
Babesiose
/
Doenças do Cão
/
Peroxirredoxinas
Limite:
Animals
Idioma:
En
Ano de publicação:
2014
Tipo de documento:
Article